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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, <t>human</t> <t>placental</t> <t>lactogen</t> <t>(hPL),</t> sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of <t>first</t> trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.
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Image Search Results


The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, human placental lactogen (hPL), sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of first trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.

Journal: Frontiers in Immunology

Article Title: Integrated Systems Biology Approach Identifies Novel Maternal and Placental Pathways of Preeclampsia

doi: 10.3389/fimmu.2018.01661

Figure Lengend Snippet: The timing of gene module dysregulation in preterm preeclampsia. (A) A database of 80,170 measurements published in 61 reports ( , , , , , – ) was built for “virtual” liquid biopsy of the placenta in preterm preeclampsia with maternal blood levels of proteins with the highest expression in the placenta among all tissues [i.e. hCG, human placental lactogen (hPL), sEng, sFlt-1, and leptin]. Levels of these biomarkers in preterm preeclampsia were expressed as the percentage of control levels (dashed line) and were represented in scatter plots by different colors reflecting gene module classification. Based on qRT-PCR data, sEng belongs to module M2 (red). hPL (M1, green module) levels in preeclampsia were constantly below control levels during gestation, while the levels of module M2 biomarkers in preeclampsia had continuous elevation compared to control levels as a function of gestational age. (B) Analysis of first trimester data revealed the lower expression of all biomarkers in preeclampsia than in controls before the onset of maternal circulation, and the increasing expression of module M2 biomarkers in preeclampsia compared to controls after 12 weeks of gestation.

Article Snippet: Concentrations of leptin and human placental lactogen (hPL) in first ( n = 12) and third ( n = 19) trimester maternal blood samples were measured with sensitive and specific immunoassays (Leptin ELISA Kit, Abnova, Taipei City, Taiwan; Human Placental Lactogen ELISA Kit, Alpco, Salem, NH, USA) according to the manufacturers’ instructions.

Techniques: Expressing, Control, Quantitative RT-PCR